TSC22D3 Kit ELISA
-
- Antigène Voir toutes TSC22D3 Kits ELISA
- TSC22D3 (TSC22 Domain Family, Member 3 (TSC22D3))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 18.52 pg/mL - 1500 pg/mL
- Seuil minimal de détection
- 18.52 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Delta-Sleep Inducing Peptide (dSIP). No significant cross-reactivity or interference between Delta-Sleep Inducing Peptide (dSIP) and analogues was observed.
- Sensibilité
- 7.15 pg/mL
- Featured
- Discover our best selling TSC22D3 Kit ELISA
- Top Product
- Discover our top product TSC22D3 Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 h
- Plaque
- Pre-coated
- Protocole
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Delta-Sleep Inducing Peptide (dSIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Delta-Sleep Inducing Peptide (dSIP) and unlabeled Delta-Sleep Inducing Peptide (dSIP) (Standards or samples) with the pre-coated antibody specific to Delta-Sleep Inducing Peptide (dSIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Delta-Sleep Inducing Peptide (dSIP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Delta-Sleep Inducing Peptide (dSIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes TSC22D3 Kits ELISA
- TSC22D3 (TSC22 Domain Family, Member 3 (TSC22D3))
- Autre désignation
- Delta-Sleep Inducing Peptide (TSC22D3 Produits)
- Synonymes
- dsipi Kit ELISA, MGC53285 Kit ELISA, zgc:65831 Kit ELISA, zgc:77028 Kit ELISA, TSC22D3 Kit ELISA, GILZ Kit ELISA, gilz Kit ELISA, tsc-22r Kit ELISA, DIP Kit ELISA, DSIPI Kit ELISA, TSC-22R Kit ELISA, hDIP Kit ELISA, Dsip1 Kit ELISA, Gilz Kit ELISA, Tilz3 Kit ELISA, Dsipi Kit ELISA, DSIP Kit ELISA, TSC22 domain family member 3 L homeolog Kit ELISA, TSC22 domain family, member 3 Kit ELISA, TSC22 domain family member 3 Kit ELISA, tsc22d3.L Kit ELISA, tsc22d3 Kit ELISA, TSC22D3 Kit ELISA, Tsc22d3 Kit ELISA
- Sujet
-
Gene Name: Delta-Sleep Inducing Peptide
Gene Aliases: DSIPI, TSC22D3, DIP, GILZ, TSC-22R, hDIP, TSC22 Domain Family Protein 3, Glucocorticoid-Induced Leucine Zipper, Delta Sleep Inducing Peptide,Immunoreactor
- ID gène
- 1831
- UniProt
- Q99576
-