DGAT1 Kit ELISA

N° du produit ABIN5653649

Détail du produit DGAT1 Kit ELISA

Antigène: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) and analogues was observed.

Sensibilité: 13.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Autre désignation: Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1 Produits)

Synonymes: ARAT Kit ELISA, ARGP1 Kit ELISA, DGAT Kit ELISA, C75990 Kit ELISA, D15Ertd23e Kit ELISA, Dgat Kit ELISA, dgat1 Kit ELISA, wu:fd36g11 Kit ELISA, zgc:77691 Kit ELISA, DGAT1 Kit ELISA, ABX45 Kit ELISA, AS11 Kit ELISA, ATDGAT Kit ELISA, DIACYLGLYCEROL ACYLTRANSFERASE Kit ELISA, F27F23.24 Kit ELISA, RDS1 Kit ELISA, TRIACYLGLYCEROL BIOSYNTHESIS DEFECT 1 Kit ELISA, DDBDRAFT_0202877 Kit ELISA, DDBDRAFT_0304727 Kit ELISA, DDB_0202877 Kit ELISA, DDB_0304727 Kit ELISA, zgc:92327 Kit ELISA, diacylglycerol O-acyltransferase 1 Kit ELISA, diacylglycerol O-acyltransferase 1a Kit ELISA, diacylglycerol O-acyltransferase 1 L homeolog Kit ELISA, membrane bound O-acyl transferase (MBOAT) family protein Kit ELISA, diacylglycerol O-acyltransferase Dga1 Kit ELISA, diacylglycerol O-acyltransferase 1b Kit ELISA, DGAT1 Kit ELISA, Dgat1 Kit ELISA, dgat1a Kit ELISA, dgat1.L Kit ELISA, TAG1 Kit ELISA, dgat1 Kit ELISA, MGYG_02131 Kit ELISA, Tsp_02502 Kit ELISA, LOC100283803 Kit ELISA, dga1 Kit ELISA, dgat1b Kit ELISA

Sujet:

Gene Name: Diacylglycerol-O-Acyltransferase Homolog 1

Gene Aliases: ARGP1, DGAT, ARAT, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase

ID gène: 8694

UniProt: O75907

Pathways: Hormone Transport