Diazepam Binding Inhibitor Kit ELISA

N° du produit ABIN5653658

Détail du produit Diazepam Binding Inhibitor Kit ELISA

Antigène: Diazepam Binding Inhibitor (DBI)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Diazepam Binding Inhibitor (DBI). No significant cross-reactivity or interference between Diazepam Binding Inhibitor (DBI) and analogues was observed.

Sensibilité: 0.135 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diazepam Binding Inhibitor (DBI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diazepam Binding Inhibitor (DBI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diazepam Binding Inhibitor (DBI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diazepam Binding Inhibitor (DBI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diazepam Binding Inhibitor (DBI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diazepam Binding Inhibitor (DBI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Diazepam Binding Inhibitor (DBI)

Autre désignation: Diazepam Binding Inhibitor (DBI Produits)

Synonymes: ACBD1 Kit ELISA, ACBP Kit ELISA, CCK-RP Kit ELISA, EP Kit ELISA, dbib Kit ELISA, DBI Kit ELISA, wu:fb63e10 Kit ELISA, zgc:56108 Kit ELISA, zgc:77734 Kit ELISA, Acbp Kit ELISA, endozepine Kit ELISA, Acoabp3 Kit ELISA, Ep Kit ELISA, Odn Kit ELISA, RNACOABP3 Kit ELISA, Ttn Kit ELISA, acbd1 Kit ELISA, acbp Kit ELISA, cck-rp Kit ELISA, dbi Kit ELISA, dbi-a Kit ELISA, dbi-b Kit ELISA, dbia Kit ELISA, diazepam binding inhibitor, acyl-CoA binding protein Kit ELISA, diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) S homeolog Kit ELISA, diazepam-binding inhibitor Kit ELISA, diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) Kit ELISA, acyl-CoA-binding protein Kit ELISA, diazepam binding inhibitor Kit ELISA, diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) L homeolog Kit ELISA, DBI Kit ELISA, dbi.S Kit ELISA, dbi Kit ELISA, LOC706367 Kit ELISA, Dbi Kit ELISA, dbi.L Kit ELISA

Sujet:

Gene Name: Diazepam Binding Inhibitor

Gene Aliases: ACBP, ACBD1, CCK-RP, EP, Endozepine, GABA Receptor Modulator, Acyl-Coenzyme A Binding Protein

ID gène: 1622

UniProt: P07108