DHODH Kit ELISA

N° du produit ABIN5653671

Détail du produit DHODH Kit ELISA

Antigène: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dihydroorotate Dehydrogenase (DHODH). No significant cross-reactivity or interference between Dihydroorotate Dehydrogenase (DHODH) and analogues was observed.

Sensibilité: 0.253 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydroorotate Dehydrogenase (DHODH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydroorotate Dehydrogenase (DHODH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydroorotate Dehydrogenase (DHODH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydroorotate Dehydrogenase (DHODH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydroorotate Dehydrogenase (DHODH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydroorotate Dehydrogenase (DHODH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Autre désignation: Dihydroorotate Dehydrogenase (DHODH Produits)

Synonymes: DHOdehase Kit ELISA, POADS Kit ELISA, URA1 Kit ELISA, 2810417D19Rik Kit ELISA, AI834883 Kit ELISA, DDBDRAFT_0167009 Kit ELISA, DDBDRAFT_0185217 Kit ELISA, DDB_0167009 Kit ELISA, DDB_0185217 Kit ELISA, DHOD Kit ELISA, DHODase Kit ELISA, XFHB_12025 Kit ELISA, dhodh Kit ELISA, dihydroorotate dehydrogenase (quinone) Kit ELISA, dihydroorotate dehydrogenase Kit ELISA, dihydroorotate dehydrogenase (quinone) L homeolog Kit ELISA, DHODH Kit ELISA, Dhodh Kit ELISA, pyrD Kit ELISA, pyr4 Kit ELISA, Mrub_0263 Kit ELISA, Arnit_2124 Kit ELISA, Trad_2703 Kit ELISA, Ftrac_1484 Kit ELISA, Ndas_0799 Kit ELISA, Mesil_1876 Kit ELISA, Slip_0841 Kit ELISA, Spirs_1436 Kit ELISA, XFHB_RS12780 Kit ELISA, dhodh.L Kit ELISA

Sujet:

Gene Name: Dihydroorotate Dehydrogenase

Gene Aliases: DHOdehase, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial

ID gène: 1723

UniProt: Q02127

Pathways: Ribonucleoside Biosynthetic Process, Protein targeting to Nucleus