DPP8 Kit ELISA

N° du produit ABIN5653687

Détail du produit DPP8 Kit ELISA

Antigène: DPP8 (Dipeptidyl-Peptidase 8 (DPP8))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 8 (DPP8). No significant cross-reactivity or interference between Dipeptidyl Peptidase 8 (DPP8) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 8 (DPP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 8 (DPP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 8 (DPP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 8 (DPP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase 8 (DPP8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase 8 (DPP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DPP8 (Dipeptidyl-Peptidase 8 (DPP8))

Autre désignation: Dipeptidyl Peptidase 8 (DPP8 Produits)

Synonymes: DP8 Kit ELISA, DPRP1 Kit ELISA, MSTP141 Kit ELISA, dp8 Kit ELISA, dpp8b Kit ELISA, dprp1 Kit ELISA, MGC131071 Kit ELISA, DPP8 Kit ELISA, dpp8 Kit ELISA, dpp8a Kit ELISA, 2310004I03Rik Kit ELISA, 4932434F09Rik Kit ELISA, AI666706 Kit ELISA, DPP VIII Kit ELISA, dipeptidyl peptidase 8 Kit ELISA, dipeptidyl-peptidase 8 L homeolog Kit ELISA, dipeptidyl-peptidase 8 Kit ELISA, dipeptidyl-peptidase 8 S homeolog Kit ELISA, dipeptidylpeptidase 8 Kit ELISA, DPP8 Kit ELISA, dpp8.L Kit ELISA, dpp8 Kit ELISA, dpp8.S Kit ELISA, Dpp8 Kit ELISA

Sujet:

Gene Name: Dipeptidyl Peptidase 8

Gene Aliases: DP8, DPRP1, MSTP141, MSTP097, MSTP135, dipeptidylpeptidase 8, Dipeptidyl Peptidase IV-Related Protein-1, Prolyl Dipeptidase DPP VIII

ID gène: 54878

UniProt: Q6V1X1