MPP3 Kit ELISA
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- Antigène Tous les produits MPP3
- MPP3 (Membrane Protein, Palmitoylated 3 (MAGUK P55 Subfamily Member 3) (MPP3))
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Discs, Large Homolog 3 (DLG3). No significant cross-reactivity or interference between Discs, Large Homolog 3 (DLG3) and analogues was observed.
- Sensibilité
- 0.115 ng/mL
- Featured
- Discover our best selling DLG3 Kit ELISA
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discs, Large Homolog 3 (DLG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discs, Large Homolog 3 (DLG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discs, Large Homolog 3 (DLG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Discs, Large Homolog 3 (DLG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Discs, Large Homolog 3 (DLG3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Discs, Large Homolog 3 (DLG3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Tous les produits MPP3
- MPP3 (Membrane Protein, Palmitoylated 3 (MAGUK P55 Subfamily Member 3) (MPP3))
- Autre désignation
- Discs, Large Homolog 3 (MPP3 Produits)
- Synonymes
- DLG3 Kit ELISA, 6430514B01 Kit ELISA, Dlgh3 Kit ELISA, CSG18 Kit ELISA, Dlg3 Kit ELISA, Dusp3 Kit ELISA, si:ch73-368i2.1 Kit ELISA, membrane palmitoylated protein 3 Kit ELISA, membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) Kit ELISA, membrane protein, palmitoylated 3a (MAGUK p55 subfamily member 3) Kit ELISA, MPP3 Kit ELISA, Mpp3 Kit ELISA, mpp3a Kit ELISA
- Sujet
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Gene Name: Discs, Large Homolog 3
Gene Aliases: MRX, XLMR, MRX90, NE-Dlg, NEDLG, SAP102, Neuroendocrine-Dlg, Synapse-associated protein 102
- ID gène
- 1741
- UniProt
- Q92796
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