RAD50 Kit ELISA

N° du produit ABIN5653712

Détail du produit RAD50 Kit ELISA

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of DNA Repair Protein RAD50 (RAD50). No significant cross-reactivity or interference between DNA Repair Protein RAD50 (RAD50) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Repair Protein RAD50 (RAD50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Repair Protein RAD50 (RAD50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Repair Protein RAD50 (RAD50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Repair Protein RAD50 (RAD50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNA Repair Protein RAD50 (RAD50) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNA Repair Protein RAD50 (RAD50) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Autre désignation: DNA Repair Protein RAD50 (RAD50 Produits)

Synonymes: NBSLD Kit ELISA, RAD502 Kit ELISA, hRad50 Kit ELISA, Mrell Kit ELISA, Rad50l Kit ELISA, 6339 Kit ELISA, CG6339 Kit ELISA, Dmel\\CG6339 Kit ELISA, Dmrad50 Kit ELISA, RAD50 Kit ELISA, Rad50 Kit ELISA, ATRAD50 Kit ELISA, DNA REPAIR-RECOMBINATION PROTEIN Kit ELISA, F22D22.28 Kit ELISA, F22D22_28 Kit ELISA, SPAP4C9.01c Kit ELISA, im:6906849 Kit ELISA, si:ch211-79h18.3 Kit ELISA, RAD50 double strand break repair protein Kit ELISA, CG6339 gene product from transcript CG6339-RE Kit ELISA, RAD50 homolog, double strand break repair protein L homeolog Kit ELISA, rad50 Kit ELISA, DNA repair protein RAD50 Kit ELISA, RAD50 homolog, double strand break repair protein Kit ELISA, DNA repair-recombination protein (RAD50) Kit ELISA, MRX complex DNA-binding subunit Kit ELISA, DNA repair protein Rad50 Kit ELISA, RAD50 recombination protein Kit ELISA, Rad50 DNA repair/recombination protein Kit ELISA, DNA repair protein rad-50 Kit ELISA, RAD50 Kit ELISA, Rad50 Kit ELISA, rad50 Kit ELISA, rad50.L Kit ELISA, LOC658288 Kit ELISA, LOC726642 Kit ELISA, rad-50 Kit ELISA

Sujet:

Gene Name: DNA Repair Protein RAD50

UniProt: P70388

Pathways: Réparation de l'ADN, Protein targeting to Nucleus