DCLK1 Kit ELISA

N° du produit ABIN5653736

Détail du produit DCLK1 Kit ELISA

Antigène: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Doublecortin Like Kinase 1 (DCLK1). No significant cross-reactivity or interference between Doublecortin Like Kinase 1 (DCLK1) and analogues was observed.

Sensibilité: 0.235 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Doublecortin Like Kinase 1 (DCLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Doublecortin Like Kinase 1 (DCLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Doublecortin Like Kinase 1 (DCLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Doublecortin Like Kinase 1 (DCLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Autre désignation: Doublecortin Like Kinase 1 (DCLK1 Produits)

Synonymes: DCLK1 Kit ELISA, DCAMKL1 Kit ELISA, dcamkl1 Kit ELISA, dclk1 Kit ELISA, wu:fc51f11 Kit ELISA, zgc:153709 Kit ELISA, MGC145348 Kit ELISA, CL1 Kit ELISA, CLICK1 Kit ELISA, DCDC3A Kit ELISA, DCLK Kit ELISA, 1700113D08Rik Kit ELISA, 2810480F11Rik Kit ELISA, AI836758 Kit ELISA, Click-I Kit ELISA, Cpg16 Kit ELISA, Dcamkl1 Kit ELISA, Dcl Kit ELISA, Dclk Kit ELISA, mKIAA0369 Kit ELISA, Ania4 Kit ELISA, doublecortin like kinase 1 Kit ELISA, doublecortin-like kinase 1a Kit ELISA, serine/threonine-protein kinase DCLK1 Kit ELISA, doublecortin-like kinase 2 Kit ELISA, doublecortin-like kinase 1 Kit ELISA, DCLK1 Kit ELISA, dclk1a Kit ELISA, LOC587664 Kit ELISA, dclk2 Kit ELISA, LOC100539645 Kit ELISA, Dclk1 Kit ELISA

Sujet:

Gene Name: Doublecortin Like Kinase 1

Gene Aliases: DCDC3A, DCLK, DCAMKL1, Doublecortin And CaM Kinase-Like 1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1

ID gène: 9201

UniProt: O15075