Dysferlin Kit ELISA

N° du produit ABIN5653759

Détail du produit Dysferlin Kit ELISA

Antigène: Dysferlin (DYSF)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dysferlin (DYSF). No significant cross-reactivity or interference between Dysferlin (DYSF) and analogues was observed.

Sensibilité: 12.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dysferlin (DYSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dysferlin (DYSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dysferlin (DYSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dysferlin (DYSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dysferlin (DYSF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dysferlin (DYSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Dysferlin (DYSF)

Autre désignation: Dysferlin (DYSF Produits)

Synonymes: DYSF Kit ELISA, fb73b05 Kit ELISA, wu:fb73b05 Kit ELISA, si:rp71-50c18.1 Kit ELISA, DKFZp459E1226 Kit ELISA, 2310004N10Rik Kit ELISA, AI604795 Kit ELISA, D6Pas3 Kit ELISA, mFLJ00175 Kit ELISA, FER1L1 Kit ELISA, LGMD2B Kit ELISA, MMD1 Kit ELISA, dysferlin Kit ELISA, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) Kit ELISA, myoferlin Kit ELISA, DYSF Kit ELISA, dysf Kit ELISA, LOC589501 Kit ELISA, Dysf Kit ELISA

Sujet:

Gene Name: Dysferlin

Gene Aliases: FER1L1, LGMD2B, Dysferlin,Limb Girdle Muscular Dystrophy 2B(Autosomal Recessive), Fer-1-like protein 1, Dystrophy-associated fer-1-like protein

ID gène: 8291

UniProt: O75923