E2F1 Kit ELISA

N° du produit ABIN5653768

Détail du produit E2F1 Kit ELISA

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 g/mL - 10 g/mL

Seuil minimal de détection: 0.156 g/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of E2F Transcription Factor 1 (E2F1). No significant cross-reactivity or interference between E2F Transcription Factor 1 (E2F1) and analogues was observed.

Sensibilité: 0.054 g/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 1 (E2F1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F Transcription Factor 1 (E2F1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 1 (E2F1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 1 (E2F1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E2F Transcription Factor 1 (E2F1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E2F Transcription Factor 1 (E2F1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: E2F Transcription Factor 1 (E2F1 Produits)

Synonymes: CG6376 Kit ELISA, DRTF1/E2F Kit ELISA, DmE2F-1 Kit ELISA, Dmel\\CG6376 Kit ELISA, Dp Kit ELISA, E(Sev-CycE)3A Kit ELISA, E(var)3-93E Kit ELISA, E(var)3-95E Kit ELISA, E(var)93E Kit ELISA, E2F Kit ELISA, E2F-1 Kit ELISA, E2F1 Kit ELISA, E2f1 Kit ELISA, Evar(3)164 Kit ELISA, dE2F Kit ELISA, dE2F1 Kit ELISA, dE2f Kit ELISA, dE2f1 Kit ELISA, de2f1 Kit ELISA, def21 Kit ELISA, drosE2F1 Kit ELISA, e2f Kit ELISA, e2f1 Kit ELISA, l(3)07172 Kit ELISA, l(3)j3B1 Kit ELISA, l(3)j3C2 Kit ELISA, l(3)rM729 Kit ELISA, RBAP1 Kit ELISA, RBBP3 Kit ELISA, RBP3 Kit ELISA, mKIAA4009 Kit ELISA, xE2F Kit ELISA, ATE2FB Kit ELISA, E2F TRANSCRIPTION FACTOR-1 E2F1 Kit ELISA, E2F transcription factor 1 Kit ELISA, E2FB Kit ELISA, E2FB TRANSCRIPTION FACTOR Kit ELISA, E2F transcription factor 1 Kit ELISA, E2F transcription factor 1 L homeolog Kit ELISA, E2f1 Kit ELISA, E2F1 Kit ELISA, e2f1.L Kit ELISA

Sujet:

Gene Name: E2F Transcription Factor 1

Gene Aliases: RBBP3, RBP3, RBAP-1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy