EML2 Kit ELISA

N° du produit ABIN5653774

Détail du produit EML2 Kit ELISA

Antigène: EML2 (Echinoderm Microtubule Associated Protein Like 2 (EML2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). No significant cross-reactivity or interference between Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Echinoderm Microtubule Associated Protein Like Protein 2 (EML2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EML2 (Echinoderm Microtubule Associated Protein Like 2 (EML2))

Autre désignation: Echinoderm Microtubule Associated Protein Like Protein 2 (EML2 Produits)

Synonymes: ELP70 Kit ELISA, EMAP-2 Kit ELISA, EMAP2 Kit ELISA, Emap Kit ELISA, Emap-2 Kit ELISA, Eml1 Kit ELISA, 1600029N02Rik Kit ELISA, wu:fq15f04 Kit ELISA, zgc:110445 Kit ELISA, eml2 Kit ELISA, echinoderm microtubule associated protein like 2 Kit ELISA, echinoderm microtubule associated protein like 2 L homeolog Kit ELISA, EML2 Kit ELISA, Eml2 Kit ELISA, eml2 Kit ELISA, eml2.L Kit ELISA

Sujet:

Gene Name: Echinoderm Microtubule Associated Protein Like Protein 2

Gene Aliases: EMAP2, ELP70, Echinoderm MT-Associated Protein (EMAP)-Like Protein 70

ID gène: 24139

UniProt: O95834

Pathways: Dynamique des Microtubules, Sensory Perception of Sound