ETFA Kit ELISA

N° du produit ABIN5653816

Détail du produit ETFA Kit ELISA

Antigène: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Alpha Polypeptide (ETFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Autre désignation: Electron Transfer Flavoprotein Alpha Polypeptide (ETFA Produits)

Synonymes: 2010200I21Rik Kit ELISA, D9Ertd394e Kit ELISA, ETF Kit ELISA, EMA Kit ELISA, GA2 Kit ELISA, MADD Kit ELISA, cb1020 Kit ELISA, fd06h11 Kit ELISA, wu:fd06h11 Kit ELISA, ema Kit ELISA, ga2 Kit ELISA, madd Kit ELISA, electron transfer flavoprotein alpha subunit Kit ELISA, electron-transfer-flavoprotein, alpha polypeptide Kit ELISA, putative electron-transfer-flavoprotein,alpha polypeptide Kit ELISA, electron transfer flavoprotein subunit alpha, mitochondrial Kit ELISA, electron transferring flavoprotein, alpha polypeptide Kit ELISA, electron transfer flavoprotein alpha subunit L homeolog Kit ELISA, ETFA Kit ELISA, Tc00.1047053503559.109 Kit ELISA, Tc00.1047053511693.90 Kit ELISA, LMJF_28_1140 Kit ELISA, LOC100194695 Kit ELISA, etfa Kit ELISA, Etfa Kit ELISA, etfa.L Kit ELISA

Sujet:

Gene Name: Electron Transfer Flavoprotein Alpha Polypeptide

Gene Aliases: EMA, GA2, MADD, Glutaric Aciduria II

ID gène: 2108

UniProt: P13804

Pathways: SARS-CoV-2 Protein Interactome