COL18A1 Kit ELISA
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- Antigène Voir toutes COL18A1 Kits ELISA
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 6.25 ng/mL - 400 ng/mL
- Seuil minimal de détection
- 6.25 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Endostatin (ES). No significant cross-reactivity or interference between Endostatin (ES) and analogues was observed.
- Sensibilité
- 2.63 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endostatin (ES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endostatin (ES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endostatin (ES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endostatin (ES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endostatin (ES) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endostatin (ES) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes COL18A1 Kits ELISA
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- Autre désignation
- Endostatin (COL18A1 Produits)
- Synonymes
- KNO Kit ELISA, KNO1 Kit ELISA, KS Kit ELISA, cb371 Kit ELISA, cb529 Kit ELISA, fc96d06 Kit ELISA, sb:cb529 Kit ELISA, wu:fb47h10 Kit ELISA, wu:fb77g12 Kit ELISA, wu:fc50f04 Kit ELISA, wu:fc96d06 Kit ELISA, wu:fk02h12 Kit ELISA, kno Kit ELISA, kno1 Kit ELISA, collagen type XVIII alpha 1 chain Kit ELISA, collagen type XVIII alpha 1 chain a Kit ELISA, collagen, type XVIII, alpha 1 Kit ELISA, collagen type XVIII alpha 1 Kit ELISA, COL18A1 Kit ELISA, col18a1a Kit ELISA, Col18a1 Kit ELISA, col18a1 Kit ELISA
- Classe de substances
- Chemical
- Sujet
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Gene Name: Endostatin
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