ECE1 Kit ELISA

N° du produit ABIN5653866

Détail du produit ECE1 Kit ELISA

Antigène: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Endothelin Converting Enzyme 1 (ECE1). No significant cross-reactivity or interference between Endothelin Converting Enzyme 1 (ECE1) and analogues was observed.

Sensibilité: 11.8 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin Converting Enzyme 1 (ECE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelin Converting Enzyme 1 (ECE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelin Converting Enzyme 1 (ECE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelin Converting Enzyme 1 (ECE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin Converting Enzyme 1 (ECE1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin Converting Enzyme 1 (ECE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Autre désignation: Endothelin Converting Enzyme 1 (ECE1 Produits)

Synonymes: ECE Kit ELISA, Ece Kit ELISA, AW322500 Kit ELISA, ECE-1 Kit ELISA, ECE-1a Kit ELISA, ECE-1b Kit ELISA, ECE-1d Kit ELISA, ece1 Kit ELISA, MGC52874 Kit ELISA, MGC130791 Kit ELISA, fd11b07 Kit ELISA, wu:fd11b07 Kit ELISA, zgc:154079 Kit ELISA, endothelin converting enzyme 1 Kit ELISA, endothelin converting enzyme like 1 Kit ELISA, endothelin converting enzyme 1 L homeolog Kit ELISA, endothelin-converting enzyme 1 Kit ELISA, Endothelin-converting enzyme 1 Kit ELISA, ECE1 Kit ELISA, ECEL1 Kit ELISA, Ece1 Kit ELISA, ece1.L Kit ELISA, Za10_0551 Kit ELISA, Arch_0474 Kit ELISA, Closa_0961 Kit ELISA, Odosp_0578 Kit ELISA, Bacsa_1787 Kit ELISA, Weevi_2017 Kit ELISA, Fluta_3444 Kit ELISA, Sphch_2390 Kit ELISA, ece1 Kit ELISA

Sujet:

Gene Name: Endothelin Converting Enzyme 1

ID gène: 1889

UniProt: P42892

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling