EZH2 Kit ELISA

N° du produit ABIN5653876

Détail du produit EZH2 Kit ELISA

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Enhancer Of Zeste Homolog 2 (EZH2). No significant cross-reactivity or interference between Enhancer Of Zeste Homolog 2 (EZH2) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enhancer Of Zeste Homolog 2 (EZH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enhancer Of Zeste Homolog 2 (EZH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enhancer Of Zeste Homolog 2 (EZH2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enhancer Of Zeste Homolog 2 (EZH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Autre désignation: Enhancer Of Zeste Homolog 2 (EZH2 Produits)

Synonymes: ENX-1 Kit ELISA, ENX1 Kit ELISA, EZH1 Kit ELISA, EZH2b Kit ELISA, KMT6 Kit ELISA, KMT6A Kit ELISA, WVS Kit ELISA, WVS2 Kit ELISA, eya2 Kit ELISA, im:7149338 Kit ELISA, zgc:136376 Kit ELISA, zgc:152758 Kit ELISA, EZH2 Kit ELISA, enx-1 Kit ELISA, enx1 Kit ELISA, ezh1 Kit ELISA, kmt6 Kit ELISA, xez Kit ELISA, Enx-1 Kit ELISA, Enx1h Kit ELISA, mKIAA4065 Kit ELISA, ezh2 Kit ELISA, olezh2 Kit ELISA, enhancer of zeste 2 polycomb repressive complex 2 subunit Kit ELISA, enhancer of zeste 2 polycomb repressive complex 2 subunit L homeolog Kit ELISA, enhancer of zeste 2 polycomb repressive complex 2 subunit S homeolog Kit ELISA, EZH2 Kit ELISA, ezh2 Kit ELISA, Ezh2 Kit ELISA, ezh2.L Kit ELISA, ezh2.S Kit ELISA

Sujet:

Gene Name: Enhancer Of Zeste Homolog 2

Gene Aliases: ENX-1, KMT6, KMT6A, Histone-lysine N-methyltransferase EZH2, Lysine N-methyltransferase 6

ID gène: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation