ECH1 Kit ELISA

N° du produit ABIN5653885

Détail du produit ECH1 Kit ELISA

Antigène: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). No significant cross-reactivity or interference between Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Autre désignation: Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1 Produits)

Synonymes: HPXEL Kit ELISA, Pxel Kit ELISA, AA617331 Kit ELISA, enoyl-CoA hydratase 1, peroxisomal Kit ELISA, enoyl CoA hydratase 1, peroxisomal Kit ELISA, enoyl-CoA hydratase 1 Kit ELISA, delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Kit ELISA, enoyl-CoA hydratase 1, peroxisomal L homeolog Kit ELISA, enoyl coenzyme A hydratase 1, peroxisomal Kit ELISA, ech1 Kit ELISA, ECH1 Kit ELISA, LOC747511 Kit ELISA, ech1.L Kit ELISA, Ech1 Kit ELISA

Sujet:

Gene Name: Enoyl Coenzyme A Hydratase 1, Peroxisomal

Gene Aliases: HPXEL, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

ID gène: 1891

UniProt: Q13011

Pathways: Monocarboxylic Acid Catabolic Process