EBI3 Kit ELISA

N° du produit ABIN5653964

Détail du produit EBI3 Kit ELISA

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Epstein Barr Virus Induced Protein 3 (EBI3). No significant cross-reactivity or interference between Epstein Barr Virus Induced Protein 3 (EBI3) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epstein Barr Virus Induced Protein 3 (EBI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epstein Barr Virus Induced Protein 3 (EBI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Autre désignation: Epstein Barr Virus Induced Protein 3 (IL-27b Produits)

Synonymes: IL-27B Kit ELISA, IL27B Kit ELISA, EBI3 Kit ELISA, il-27rb Kit ELISA, si:dkey-97a13.4 Kit ELISA, EBI-3 Kit ELISA, IL-27 Kit ELISA, Epstein-Barr virus induced 3 Kit ELISA, Epstein-Barr virus induced gene 3 Kit ELISA, EBI3 Kit ELISA, ebi3 Kit ELISA, Ebi3 Kit ELISA

Classe de substances: Viral Protein

Sujet:

Gene Name: Epstein Barr Virus Induced Protein 3

Gene Aliases: IL27B, IL27-B, Interleukin 27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein

ID gène: 50498

UniProt: O35228