ESRRA Kit ELISA

N° du produit ABIN5653994

Détail du produit ESRRA Kit ELISA

Antigène: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Estrogen Related Receptor Alpha (ERRa). No significant cross-reactivity or interference between Estrogen Related Receptor Alpha (ERRa) and analogues was observed.

Sensibilité: 0.127 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Related Receptor Alpha (ERRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Related Receptor Alpha (ERRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Related Receptor Alpha (ERRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Related Receptor Alpha (ERRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Related Receptor Alpha (ERRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Related Receptor Alpha (ERRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Autre désignation: Estrogen Related Receptor Alpha (ESRRA Produits)

Synonymes: err1 Kit ELISA, erra Kit ELISA, esrral Kit ELISA, erralpha Kit ELISA, nr3b1 Kit ELISA, MGC80854 Kit ELISA, ESRRA Kit ELISA, esrl1 Kit ELISA, LOC100125507 Kit ELISA, ERRalpha Kit ELISA, Err1 Kit ELISA, Estrra Kit ELISA, Nr3b1 Kit ELISA, Errra Kit ELISA, ERR1 Kit ELISA, ERRa Kit ELISA, ESRL1 Kit ELISA, NR3B1 Kit ELISA, ESTRRA Kit ELISA, estrogen-related receptor alpha Kit ELISA, estrogen related receptor alpha L homeolog Kit ELISA, estrogen related receptor alpha Kit ELISA, catsper channel auxiliary subunit zeta Kit ELISA, estrogen related receptor, alpha Kit ELISA, esrra Kit ELISA, esrra.L Kit ELISA, ESRRA Kit ELISA, LOC100125507 Kit ELISA, CATSPERZ Kit ELISA, Esrra Kit ELISA

Sujet:

Gene Name: Estrogen Related Receptor Alpha

Gene Aliases: ERR1, ERRa, ERRalpha, ESRL1, NR3B1, Estrogen receptor-like 1, Nuclear receptor subfamily 3 group B member 1

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha