Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Estrone Kit CLIA

Reactivité: Différentes espèces Chemiluminescent Competition ELISA 23.44 pg/mL - 6000 pg/mL Plasma, Serum
N° du produit ABIN5653996
  • Antigène Voir toutes Estrone Kits CLIA
    Estrone
    Reactivité
    • 5
    • 1
    • 1
    Différentes espèces
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Competition ELISA
    Gamme de detection
    23.44 pg/mL - 6000 pg/mL
    Seuil minimal de détection
    23.44 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Estrone (E1). No significant cross-reactivity or interference between Estrone (E1) and analogues was observed.
    Sensibilité
    7.96 pg/mL
    Featured
    Discover our best selling Estrone Kit ELISA
    Top Product
    Discover our top product Estrone Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Estrone (E1). A competitive inhibition reaction is launched between biotin labeled Estrone (E1) and unlabeled Estrone (E1) (Standards or samples) with the pre-coated antibody specific to Estrone (E1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estrone (E1) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Estrone (E1) level in the sample or standard.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrone (E1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrone (E1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Estrone Kits CLIA
    Estrone
    Abstract
    Estrone Produits
    Classe de substances
    Amino Acid
    Sujet

    Gene Name: Estrone

    Gene Aliases: Oestrone

Vous êtes ici:
Support technique