XPO1 Kit ELISA

N° du produit ABIN5654020

Détail du produit XPO1 Kit ELISA

Antigène: XPO1 (Exportin 1 (XPO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Exportin 1 (XPO1). No significant cross-reactivity or interference between Exportin 1 (XPO1) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exportin 1 (XPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exportin 1 (XPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exportin 1 (XPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exportin 1 (XPO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exportin 1 (XPO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exportin 1 (XPO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: XPO1 (Exportin 1 (XPO1))

Autre désignation: Exportin 1 (XPO1 Produits)

Synonymes: CG13387 Kit ELISA, CRM1 Kit ELISA, Crm1 Kit ELISA, DCRM1 Kit ELISA, Dmel\\CG13387 Kit ELISA, Emb Kit ELISA, XPO-1 Kit ELISA, XPO1 Kit ELISA, Xpo1 Kit ELISA, crm1 Kit ELISA, dCRM1 Kit ELISA, l(2)k16715 Kit ELISA, 18.m06600 Kit ELISA, DDBDRAFT_0183812 Kit ELISA, DDBDRAFT_0234066 Kit ELISA, DDB_0183812 Kit ELISA, DDB_0234066 Kit ELISA, exportin-1 Kit ELISA, LOC100220104 Kit ELISA, emb Kit ELISA, exp1 Kit ELISA, Xpo Kit ELISA, AA420417 Kit ELISA, Exp1 Kit ELISA, embargoed Kit ELISA, exportin 1 Kit ELISA, putative exportin 1 Kit ELISA, chromosome region maintenance protein 1 Kit ELISA, exportin-1 Kit ELISA, exportin 1 (CRM1 homolog, yeast) Kit ELISA, exportin 1 S homeolog Kit ELISA, emb Kit ELISA, cgd3_3060 Kit ELISA, Tc00.1047053511725.150 Kit ELISA, Tb11.01.5940 Kit ELISA, BBOV_II007220 Kit ELISA, LMJF_32_1100 Kit ELISA, xpo1 Kit ELISA, Xpo1 Kit ELISA, XPO1 Kit ELISA, LOC100165622 Kit ELISA, LOC100633509 Kit ELISA, xpo1.S Kit ELISA

Sujet:

Gene Name: Exportin 1

Gene Aliases: Exp1, CRM1 Homolog,Yeast, Chromosome region maintenance 1 protein homolog

ID gène: 7514

UniProt: O14980

Pathways: M Phase