XPO5 Kit ELISA

N° du produit ABIN5654021

Détail du produit XPO5 Kit ELISA

Antigène: XPO5 (Exportin 5 (XPO5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Exportin 5 (XPO5). No significant cross-reactivity or interference between Exportin 5 (XPO5) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exportin 5 (XPO5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exportin 5 (XPO5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exportin 5 (XPO5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exportin 5 (XPO5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exportin 5 (XPO5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exportin 5 (XPO5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: XPO5 (Exportin 5 (XPO5))

Autre désignation: Exportin 5 (XPO5 Produits)

Synonymes: exp5 Kit ELISA, CG12234 Kit ELISA, Dmel\\CG12234 Kit ELISA, Exp-5 Kit ELISA, Exp5 Kit ELISA, RanBP21 Kit ELISA, dmExp5 Kit ELISA, 2410004H11Rik Kit ELISA, 2700038C24Rik Kit ELISA, AI648907 Kit ELISA, AW549301 Kit ELISA, RanBp21 Kit ELISA, mKIAA1291 Kit ELISA, exportin-5 Kit ELISA, ranbp21 Kit ELISA, DDBDRAFT_0185980 Kit ELISA, DDBDRAFT_0237583 Kit ELISA, DDB_0185980 Kit ELISA, DDB_0237583 Kit ELISA, XPO5 Kit ELISA, exportin 5 Kit ELISA, CG12234 gene product from transcript CG12234-RB Kit ELISA, armadillo-like helical domain-containing protein Kit ELISA, XPO5 Kit ELISA, Ranbp21 Kit ELISA, Xpo5 Kit ELISA, xpo5 Kit ELISA

Sujet:

Gene Name: Exportin 5

Gene Aliases: RANBP21, Exp5, Ran-binding protein 21

ID gène: 57510

UniProt: Q9HAV4

Pathways: Regulatory RNA Pathways, Protein targeting to Nucleus