FDPS Kit ELISA

N° du produit ABIN5654055

Détail du produit FDPS Kit ELISA

Antigène: FDPS (Farnesyl Diphosphate Synthase (FDPS))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Farnesyl Diphosphate Synthase (FDPS). No significant cross-reactivity or interference between Farnesyl Diphosphate Synthase (FDPS) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyl Diphosphate Synthase (FDPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesyl Diphosphate Synthase (FDPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesyl Diphosphate Synthase (FDPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Farnesyl Diphosphate Synthase (FDPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FDPS (Farnesyl Diphosphate Synthase (FDPS))

Autre désignation: Farnesyl Diphosphate Synthase (FDPS Produits)

Synonymes: CG12389 Kit ELISA, DmFPPS Kit ELISA, DmFPPSase Kit ELISA, DmFPS Kit ELISA, Dmel\\CG12389 Kit ELISA, FPS Kit ELISA, fpps Kit ELISA, fps Kit ELISA, l(2)k06103 Kit ELISA, v(2)k03514 Kit ELISA, NCU01175.1 Kit ELISA, 16.m00760 Kit ELISA, DDBDRAFT_0206220 Kit ELISA, DDBDRAFT_0215017 Kit ELISA, DDB_0206220 Kit ELISA, DDB_0215017 Kit ELISA, FPPS Kit ELISA, Ac2-125 Kit ELISA, (2E Kit ELISA, 6030492I17Rik Kit ELISA, AI256750 Kit ELISA, Fdpsl1 Kit ELISA, mKIAA1293 Kit ELISA, Farnesyl pyrophosphate synthase Kit ELISA, farnesyl diphosphate synthase Kit ELISA, farnesyl diphosphate synthase L homeolog Kit ELISA, farnesyl pyrophosphate synthase Kit ELISA, farnesyl pyrophosphate synthetase Kit ELISA, farnesyl-pyrophosphate synthetase Kit ELISA, FDP synthase Kit ELISA, Farnesyl pyrophosphate synthetase Kit ELISA, farnesyl diphosphate synthetase Kit ELISA, farnesyl pyrophosphate synthase 1 Kit ELISA, Fpps Kit ELISA, fdps Kit ELISA, fdps.L Kit ELISA, MGG_08649 Kit ELISA, TP03_0857 Kit ELISA, Tb09.160.4300 Kit ELISA, fpp Kit ELISA, BBOV_I000120 Kit ELISA, fps Kit ELISA, fpps Kit ELISA, FDPS Kit ELISA, Fdps Kit ELISA, fps1 Kit ELISA

Sujet:

Gene Name: Farnesyl Diphosphate Synthase

Gene Aliases: FPS, Farnesyl Pyrophosphate Synthetase, Dimethylallyltranstransferase, Geranyltranstransferase

ID gène: 83791

UniProt: P05369

Pathways: Regulation of Muscle Cell Differentiation