Fascin Kit ELISA

N° du produit ABIN5654061

Détail du produit Fascin Kit ELISA

Antigène: Fascin (FSCN1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fascin (FSCN). No significant cross-reactivity or interference between Fascin (FSCN) and analogues was observed.

Sensibilité: 0.131 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fascin (FSCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fascin (FSCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fascin (FSCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fascin (FSCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fascin (FSCN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fascin (FSCN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Fascin (FSCN1)

Autre désignation: Fascin (FSCN1 Produits)

Synonymes: CG15331 Kit ELISA, CG1536 Kit ELISA, CG32858 Kit ELISA, Dmel\\CG32858 Kit ELISA, SN Kit ELISA, Sn Kit ELISA, fs(1)A1057 Kit ELISA, fs(1)K1421 Kit ELISA, fs(1)K418 Kit ELISA, fs(1)K473 Kit ELISA, fs(1)K743 Kit ELISA, fs(1)M45 Kit ELISA, p55 Kit ELISA, snl Kit ELISA, MGC75811 Kit ELISA, fscn1 Kit ELISA, sb:cb589 Kit ELISA, zgc:153632 Kit ELISA, AI663989 Kit ELISA, Fan1 Kit ELISA, fascin-1 Kit ELISA, Fascin Kit ELISA, fscn Kit ELISA, FAN1 Kit ELISA, HSN Kit ELISA, SNL Kit ELISA, fascin Kit ELISA, singed Kit ELISA, fascin actin-bundling protein 1 Kit ELISA, fascin actin-bundling protein 1a Kit ELISA, fascin actin-bundling protein 1 L homeolog Kit ELISA, sn Kit ELISA, fscn1 Kit ELISA, fscn1a Kit ELISA, Fscn1 Kit ELISA, fscn1.L Kit ELISA, FSCN1 Kit ELISA

Sujet:

Gene Name: Fascin

Gene Aliases: FSCN1, SNL, p55, Singed-like protein, 55 kDa actin-bundling protein, Fascin homolog 1, Actin-Bundling Protein(Strongylocentrotus Purpuratus), Singed,Drosophila,Homolog-Like

ID gène: 6624

UniProt: Q16658