FAAH Kit ELISA

N° du produit ABIN5654062

Détail du produit FAAH Kit ELISA

Antigène: FAAH (Fatty Acid Amide Hydrolase (FAAH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Amide Hydrolase (FAAH). No significant cross-reactivity or interference between Fatty Acid Amide Hydrolase (FAAH) and analogues was observed.

Sensibilité: 0.58 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Amide Hydrolase (FAAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Amide Hydrolase (FAAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Amide Hydrolase (FAAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Amide Hydrolase (FAAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Amide Hydrolase (FAAH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Amide Hydrolase (FAAH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FAAH (Fatty Acid Amide Hydrolase (FAAH))

Autre désignation: Fatty Acid Amide Hydrolase (FAAH Produits)

Synonymes: AW412498 Kit ELISA, FAAH-1 Kit ELISA, FAAH Kit ELISA, faah Kit ELISA, faah-1 Kit ELISA, AtFAAH Kit ELISA, fatty acid amide hydrolase Kit ELISA, fatty acid amide hydrolase Kit ELISA, fatty-acid amide hydrolase 1 Kit ELISA, fatty-acid amide hydrolase Kit ELISA, fatty acid amide hydrolase, gene 1 S homeolog Kit ELISA, fatty acid amide hydrolase, gene 3 Kit ELISA, Faah Kit ELISA, FAAH Kit ELISA, LOC482510 Kit ELISA, faah Kit ELISA, NFIA_029220 Kit ELISA, NFIA_010900 Kit ELISA, ACLA_086700 Kit ELISA, BDBG_07484 Kit ELISA, faah.1.S Kit ELISA, Tsp_03856 Kit ELISA, faah.3 Kit ELISA

Sujet:

Gene Name: Fatty Acid Amide Hydrolase

Gene Aliases: FAAH-1, Anandamide amidohydrolase 1, Oleamide hydrolase 1

Pathways: Monocarboxylic Acid Catabolic Process