FGF12 Kit ELISA

N° du produit ABIN5654200

Détail du produit FGF12 Kit ELISA

Antigène: FGF12 (Fibroblast Growth Factor 12 (FGF12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 12 (FGF12). No significant cross-reactivity or interference between Fibroblast Growth Factor 12 (FGF12) and analogues was observed.

Sensibilité: 5.6 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 12 (FGF12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 12 (FGF12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 12 (FGF12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 12 (FGF12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 12 (FGF12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 12 (FGF12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FGF12 (Fibroblast Growth Factor 12 (FGF12))

Autre désignation: Fibroblast Growth Factor 12 (FGF12 Produits)

Synonymes: FHF1 Kit ELISA, FHF-1 Kit ELISA, MGC84150 Kit ELISA, FGF12 Kit ELISA, fgf12 Kit ELISA, zgc:153104 Kit ELISA, fhf1 Kit ELISA, fgf12b Kit ELISA, DKFZp459P139 Kit ELISA, FGF12B Kit ELISA, AV114868 Kit ELISA, FGF-12 Kit ELISA, Fhf1 Kit ELISA, Fhf1b Kit ELISA, fibroblast growth factor 12 Kit ELISA, fibroblast growth factor 12 S homeolog Kit ELISA, fibroblast growth factor 12a Kit ELISA, FGF12 Kit ELISA, fgf12.S Kit ELISA, fgf12a Kit ELISA, fgf12 Kit ELISA, Fgf12 Kit ELISA

Sujet:

Gene Name: Fibroblast Growth Factor 12

Gene Aliases: FGF12B, FHF1, Fibroblast Growth Factor Homologous Factor 1, Myocyte-Activating Factor

ID gène: 2257

UniProt: P61328

Pathways: Negative Regulation of Transporter Activity