MTOR Kit ELISA
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- Antigène Voir toutes MTOR (mTOR) Kits ELISA
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP). No significant cross-reactivity or interference between FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) and analogues was observed.
- Sensibilité
- 0.114 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes MTOR (mTOR) Kits ELISA
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- Autre désignation
- FK506 Binding Protein 12 Rapamycin Associated Protein (mTOR Produits)
- Synonymes
- FRAP1 Kit ELISA, FRAP Kit ELISA, FRAP2 Kit ELISA, RAFT1 Kit ELISA, RAPT1 Kit ELISA, 2610315D21Rik Kit ELISA, AI327068 Kit ELISA, Frap1 Kit ELISA, flat Kit ELISA, frap1 Kit ELISA, tor Kit ELISA, wu:fc22h08 Kit ELISA, mechanistic target of rapamycin kinase Kit ELISA, mechanistic target of rapamycin Kit ELISA, mechanistic target of rapamycin (serine/threonine kinase) Kit ELISA, MTOR Kit ELISA, Mtor Kit ELISA, mtor Kit ELISA
- Sujet
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Gene Name: FK506 Binding Protein 12 Rapamycin Associated Protein
Gene Aliases: FRAP1, MTOR, FRAP2, MTOR, RAFT1, RAPT1, Mammalian Target Of Rapamycin, FKBP12-Rapamycin Complex-Associated Protein 1, Rapamycin and FKBP12 target 1
- ID gène
- 2475
- UniProt
- P42345
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg
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