FOLR1 Kit ELISA

N° du produit ABIN5654379

Détail du produit FOLR1 Kit ELISA

Antigène: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Folate Receptor 1, Adult (FOLR1). No significant cross-reactivity or interference between Folate Receptor 1, Adult (FOLR1) and analogues was observed.

Sensibilité: 5.8 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Folate Receptor 1, Adult (FOLR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Folate Receptor 1, Adult (FOLR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Folate Receptor 1, Adult (FOLR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Folate Receptor 1, Adult (FOLR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Autre désignation: Folate Receptor 1, Adult (FOLR1 Produits)

Synonymes: FBP Kit ELISA, FOLR Kit ELISA, fbp Kit ELISA, folr Kit ELISA, folr1 Kit ELISA, FBP1 Kit ELISA, Folbp-1 Kit ELISA, Folbp1 Kit ELISA, Fbp1 Kit ELISA, FOLR1 Kit ELISA, Folr1 Kit ELISA, folate receptor 1 Kit ELISA, folate receptor 1 (adult) Kit ELISA, folate receptor 1 (adult) L homeolog Kit ELISA, zgc:165502 Kit ELISA, Folate receptor alpha Kit ELISA, folate receptor alpha Kit ELISA, FOLR1 Kit ELISA, folr1 Kit ELISA, folr1.L Kit ELISA, zgc:165502 Kit ELISA, Folr1 Kit ELISA, LOC100066084 Kit ELISA, LOC100725439 Kit ELISA

Sujet:

Gene Name: Folate Receptor 1, Adult

Gene Aliases: FBP, FOLR, FR-alpha, MOv18, Adult folate-binding protein, KB cells FBP, Ovarian tumor-associated antigen MOv18

ID gène: 2348

UniProt: P15328

Pathways: Dicarboxylic Acid Transport