FOXP1 Kit ELISA

N° du produit ABIN5654411

Détail du produit FOXP1 Kit ELISA

Antigène: FOXP1 (Forkhead Box P1 (FOXP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein P1 (FOXP1). No significant cross-reactivity or interference between Forkhead Box Protein P1 (FOXP1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P1 (FOXP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein P1 (FOXP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein P1 (FOXP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein P1 (FOXP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein P1 (FOXP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein P1 (FOXP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FOXP1 (Forkhead Box P1 (FOXP1))

Autre désignation: Forkhead Box Protein P1 (FOXP1 Produits)

Synonymes: 12CC4 Kit ELISA, QRF1 Kit ELISA, hFKH1B Kit ELISA, 3110052D19Rik Kit ELISA, 4932443N09Rik Kit ELISA, AI461938 Kit ELISA, AW494214 Kit ELISA, foxp1 Kit ELISA, im:7139058 Kit ELISA, wu:fc83a06 Kit ELISA, FOXP1 Kit ELISA, MGC145686 Kit ELISA, 12cc4 Kit ELISA, fkh1b Kit ELISA, hspc215 Kit ELISA, qrf1 Kit ELISA, xlfoxp1 Kit ELISA, im:7146611 Kit ELISA, wu:fb97f01 Kit ELISA, zgc:154010 Kit ELISA, forkhead box P1 Kit ELISA, forkhead box P1b Kit ELISA, forkhead box P1 S homeolog Kit ELISA, forkhead box P1a Kit ELISA, FOXP1 Kit ELISA, Foxp1 Kit ELISA, foxp1b Kit ELISA, foxp1 Kit ELISA, foxp1.S Kit ELISA, foxp1a Kit ELISA

ID gène: 27086

UniProt: Q9H334

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response