FOXP3 Kit ELISA
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- Antigène Voir toutes FOXP3 Kits ELISA
- FOXP3 (Forkhead Box P3 (FOXP3))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein P3 (FOXP3). No significant cross-reactivity or interference between Forkhead Box Protein P3 (FOXP3) and analogues was observed.
- Sensibilité
- 0.117 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P3 (FOXP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein P3 (FOXP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein P3 (FOXP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein P3 (FOXP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein P3 (FOXP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein P3 (FOXP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes FOXP3 Kits ELISA
- FOXP3 (Forkhead Box P3 (FOXP3))
- Autre désignation
- Forkhead Box Protein P3 (FOXP3 Produits)
- Synonymes
- foxp3 Kit ELISA, FOXP3 Kit ELISA, AIID Kit ELISA, DIETER Kit ELISA, IPEX Kit ELISA, PIDX Kit ELISA, XPID Kit ELISA, JM2 Kit ELISA, scurfin Kit ELISA, sf Kit ELISA, RGD1562112 Kit ELISA, forkhead box P3 Kit ELISA, forkhead box P3a Kit ELISA, forkhead box P3 L homeolog Kit ELISA, FOXP3 Kit ELISA, foxp3a Kit ELISA, foxp3 Kit ELISA, foxp3.L Kit ELISA, Foxp3 Kit ELISA
- Sujet
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Gene Name: Forkhead Box Protein P3
Gene Aliases: FOX-P3, AIID, DIETER, IPEX, JM2, PIDX, XPID, SCURFIN, Immune Dysregulation,Polyendocrinopathy,Enteropathy,X-Linked
- ID gène
- 20371
- UniProt
- Q99JB6
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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