FUCA2 Kit ELISA

N° du produit ABIN5654438

Détail du produit FUCA2 Kit ELISA

Antigène: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fucosidase Alpha L2, Plasma (FUCa2). No significant cross-reactivity or interference between Fucosidase Alpha L2, Plasma (FUCa2) and analogues was observed.

Sensibilité: 6.8 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosidase Alpha L2, Plasma (FUCa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosidase Alpha L2, Plasma (FUCa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosidase Alpha L2, Plasma (FUCa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosidase Alpha L2, Plasma (FUCa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Autre désignation: Fucosidase Alpha L2, Plasma (FUCA2 Produits)

Synonymes: MGC68702 Kit ELISA, zgc:92013 Kit ELISA, wu:fc20c06 Kit ELISA, F10M10.30 Kit ELISA, F10M10_30 Kit ELISA, SMb20490 Kit ELISA, dJ20N2.5 Kit ELISA, 0610025O11Rik Kit ELISA, 5530401P20Rik Kit ELISA, alpha-L-fucosidase 2 L homeolog Kit ELISA, fucosidase, alpha-L- 2, plasma Kit ELISA, alpha-L-fucosidase 2 Kit ELISA, 1,2-alpha-L-fucosidase Kit ELISA, methylthioribulose-1-phosphate dehydratase Kit ELISA, fuculose-1-phosphate aldolase Kit ELISA, L-fuculose phosphate aldolase Kit ELISA, fuca2.L Kit ELISA, FUCA2 Kit ELISA, fuca2 Kit ELISA, FUC95A Kit ELISA, LOC100282758 Kit ELISA, LOC100284864 Kit ELISA, LOC100284892 Kit ELISA, LOC100285541 Kit ELISA, LOC100285863 Kit ELISA, LOC100286039 Kit ELISA, fucA2 Kit ELISA, Fuca2 Kit ELISA

Sujet:

Gene Name: Fucosidase Alpha L2, Plasma

Gene Aliases: Alpha-L-fucoside fucohydrolase 2

ID gène: 2519

UniProt: Q9BTY2