FAH Kit ELISA

N° du produit ABIN5654443

Détail du produit FAH Kit ELISA

Antigène: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fumarylacetoacetate Hydrolase (FAH). No significant cross-reactivity or interference between Fumarylacetoacetate Hydrolase (FAH) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fumarylacetoacetate Hydrolase (FAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fumarylacetoacetate Hydrolase (FAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fumarylacetoacetate Hydrolase (FAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fumarylacetoacetate Hydrolase (FAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fumarylacetoacetate Hydrolase (FAH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fumarylacetoacetate Hydrolase (FAH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Autre désignation: Fumarylacetoacetate Hydrolase (FAH Produits)

Synonymes: CG14993 Kit ELISA, Dmel\\CG14993 Kit ELISA, dfaa Kit ELISA, l(3)64Al Kit ELISA, l(3)SH11 Kit ELISA, PSPTO3550 Kit ELISA, fb59b12 Kit ELISA, wu:fb59b12 Kit ELISA, zgc:55316 Kit ELISA, FAA Kit ELISA, Fumarylacetoacetase Kit ELISA, FumarylAcetoacetate Hydrolase Kit ELISA, fumarylacetoacetase Kit ELISA, fumarylacetoacetate hydrolase Kit ELISA, fumarylacetoacetate hydrolase (fumarylacetoacetase) Kit ELISA, fumarylacetoacetate hydrolase (fumarylacetoacetase) L homeolog Kit ELISA, Faa Kit ELISA, fah-1 Kit ELISA, fahA Kit ELISA, hmgB Kit ELISA, Ndas_1870 Kit ELISA, Lbys_3503 Kit ELISA, Riean_0663 Kit ELISA, Ftrac_0820 Kit ELISA, Celal_3675 Kit ELISA, Celly_2655 Kit ELISA, Weevi_0208 Kit ELISA, Fluta_2435 Kit ELISA, Halhy_2019 Kit ELISA, Lacal_2931 Kit ELISA, FsymDg_1892 Kit ELISA, Fah Kit ELISA, fah Kit ELISA, fah.L Kit ELISA, FAH Kit ELISA

Sujet:

Gene Name: Fumarylacetoacetate Hydrolase

Gene Aliases: FAA, Fumarylacetoacetase, Tyrosinemia 1, Beta-diketonase

ID gène: 2184

UniProt: P16930