GABARAPL2 Kit ELISA

N° du produit ABIN5654464

Détail du produit GABARAPL2 Kit ELISA

Antigène: GABARAPL2 (GABA(A) Receptor-Associated Protein-Like 2 (GABARAPL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). No significant cross-reactivity or interference between GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GABARAPL2 (GABA(A) Receptor-Associated Protein-Like 2 (GABARAPL2))

Autre désignation: GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2 Produits)

Synonymes: GABARAPL2 Kit ELISA, atg8 Kit ELISA, gef2 Kit ELISA, gate16 Kit ELISA, gate-16 Kit ELISA, Gef2 Kit ELISA, ATG8 Kit ELISA, ATG8C Kit ELISA, GATE-16 Kit ELISA, GATE16 Kit ELISA, GEF-2 Kit ELISA, GEF2 Kit ELISA, zgc:92319 Kit ELISA, 0610012F20Rik Kit ELISA, 2900019O08Rik Kit ELISA, AI173605 Kit ELISA, GABA type A receptor associated protein like 2 Kit ELISA, GABA(A) receptor-associated protein like 2 Kit ELISA, GABA(A) receptor-associated protein like 2 L homeolog Kit ELISA, gamma-aminobutyric acid (GABA) A receptor-associated protein-like 2 Kit ELISA, GABARAPL2 Kit ELISA, gabarapl2 Kit ELISA, Gabarapl2 Kit ELISA, gabarapl2.L Kit ELISA

Sujet:

Gene Name: GABA-A Receptor Associated Protein Like Protein 2

Gene Aliases: ATG8, GATE16, GEF2, FLC3A, General protein transport factor p16, Ganglioside expression factor 2, Golgi-associated ATPase enhancer of 16 kDa, MAP1 light chain 3-related

ID gène: 11345

UniProt: P60520

Pathways: Autophagy