GALT Kit ELISA

N° du produit ABIN5654467

Détail du produit GALT Kit ELISA

Antigène: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Galactose-1-Phosphate Uridylyltransferase (GALT). No significant cross-reactivity or interference between Galactose-1-Phosphate Uridylyltransferase (GALT) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactose-1-Phosphate Uridylyltransferase (GALT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactose-1-Phosphate Uridylyltransferase (GALT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactose-1-Phosphate Uridylyltransferase (GALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactose-1-Phosphate Uridylyltransferase (GALT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactose-1-Phosphate Uridylyltransferase (GALT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactose-1-Phosphate Uridylyltransferase (GALT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Autre désignation: Galactose-1-Phosphate Uridylyltransferase (GALT Produits)

Synonymes: GALT Kit ELISA, AW553376 Kit ELISA, CG9232 Kit ELISA, Dmel\\CG9232 Kit ELISA, dGALT Kit ELISA, galactose-1-phosphate uridylyltransferase Kit ELISA, galactose-1-phosphate uridylyltransferase, GalT Kit ELISA, probable galactose-1-phosphate uridylyltransferase galtb [second part] Kit ELISA, UDP-glucose--galactose-1-phosphate uridylyltransferase Kit ELISA, GalT galactose-1-phosphate uridylyltransferase Kit ELISA, galactose-1-phosphate uridyl transferase Kit ELISA, Galactose-1-phosphate uridylyltransferase Kit ELISA, galactose-1-phosphate uridylyltransferase S homeolog Kit ELISA, GALT Kit ELISA, galT Kit ELISA, Galt Kit ELISA, CNM00620 Kit ELISA, Mrub_2813 Kit ELISA, Mesil_2395 Kit ELISA, Trad_0989 Kit ELISA, Igag_1846 Kit ELISA, VDIS_RS10425 Kit ELISA, Intca_2810 Kit ELISA, Marky_2157 Kit ELISA, Selsp_1876 Kit ELISA, Trebr_2368 Kit ELISA, Halhy_5538 Kit ELISA, Theth_1357 Kit ELISA, galt.S Kit ELISA

Sujet:

Gene Name: Galactose-1-Phosphate Uridylyltransferase

Gene Aliases: UDP-glucose--hexose-1-phosphate uridylyltransferase

ID gène: 298003

UniProt: P43424