GGH Kit ELISA

N° du produit ABIN5654503

Détail du produit GGH Kit ELISA

Antigène: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Gamma-Glutamyl Hydrolase (gGH). No significant cross-reactivity or interference between Gamma-Glutamyl Hydrolase (gGH) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyl Hydrolase (gGH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Glutamyl Hydrolase (gGH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyl Hydrolase (gGH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-Glutamyl Hydrolase (gGH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Glutamyl Hydrolase (gGH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Glutamyl Hydrolase (gGH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Autre désignation: Gamma-Glutamyl Hydrolase (GGH Produits)

Synonymes: CK2A2 Kit ELISA, CSNK2A1 Kit ELISA, GH Kit ELISA, gamma-GH Kit ELISA, zgc:76958 Kit ELISA, wu:fa56a05 Kit ELISA, wu:fj90a01 Kit ELISA, MGC84044 Kit ELISA, GGH Kit ELISA, MGC130662 Kit ELISA, ATGGH2 Kit ELISA, gamma-glutamyl hydrolase 2 Kit ELISA, casein kinase 2 alpha 2 Kit ELISA, gamma-glutamyl hydrolase Kit ELISA, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) Kit ELISA, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) L homeolog Kit ELISA, gamma-glutamyl hydrolase 2 Kit ELISA, Gamma-glutamyl hydrolase Kit ELISA, CSNK2A2 Kit ELISA, GGH Kit ELISA, Ggh Kit ELISA, ggh Kit ELISA, ggh.L Kit ELISA, ggh.2.L Kit ELISA, GGH2 Kit ELISA, GH Kit ELISA

Sujet:

Gene Name: Gamma-Glutamyl Hydrolase

Gene Aliases: GH, conjugase, folylpolygammaglutamyl hydrolase, Gamma-Glu-X carboxypeptidase

ID gène: 8836

UniProt: Q92820

Pathways: SARS-CoV-2 Protein Interactome