GAD Kit ELISA

N° du produit ABIN5654648

Détail du produit GAD Kit ELISA

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutamate Decarboxylase 1, Brain (GAD1). No significant cross-reactivity or interference between Glutamate Decarboxylase 1, Brain (GAD1) and analogues was observed.

Sensibilité: 0.31 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 1, Brain (GAD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Decarboxylase 1, Brain (GAD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Decarboxylase 1, Brain (GAD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Decarboxylase 1, Brain (GAD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Decarboxylase 1, Brain (GAD1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Decarboxylase 1, Brain (GAD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Autre désignation: Glutamate Decarboxylase 1, Brain (GAD1 Produits)

Synonymes: CPSQ1 Kit ELISA, GAD Kit ELISA, SCP Kit ELISA, GAD-67 Kit ELISA, cpsq1 Kit ELISA, gad1 Kit ELISA, gad1-A Kit ELISA, gad67 Kit ELISA, GAD67 Kit ELISA, GAD1 Kit ELISA, GLUTAMATE DECARBOXYLASE Kit ELISA, GLUTAMATE DECARBOXYLASE 1 Kit ELISA, MKP11.30 Kit ELISA, MKP11_30 Kit ELISA, glutamate decarboxylase Kit ELISA, EP10 Kit ELISA, GAD25 Kit ELISA, GAD44 Kit ELISA, Gad-1 Kit ELISA, Gad67 Kit ELISA, glutamate decarboxylase 1 Kit ELISA, glutamate decarboxylase 1 L homeolog Kit ELISA, glutamate decarboxylase Kit ELISA, glutamate decarboxylase 1b Kit ELISA, GAD1 Kit ELISA, gad1.1.L Kit ELISA, GAD Kit ELISA, Gad1 Kit ELISA, gad1b Kit ELISA

Sujet:

Gene Name: Glutamate Decarboxylase 1, Brain

Gene Aliases: GAD67, SCP, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform

UniProt: P48318