QPCT Kit ELISA

N° du produit ABIN5654685

Détail du produit QPCT Kit ELISA

Antigène: QPCT (Glutaminyl-Peptide Cyclotransferase (QPCT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutaminyl Peptide Cyclotransferase (QPCT). No significant cross-reactivity or interference between Glutaminyl Peptide Cyclotransferase (QPCT) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaminyl Peptide Cyclotransferase (QPCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaminyl Peptide Cyclotransferase (QPCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaminyl Peptide Cyclotransferase (QPCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutaminyl Peptide Cyclotransferase (QPCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutaminyl Peptide Cyclotransferase (QPCT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutaminyl Peptide Cyclotransferase (QPCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: QPCT (Glutaminyl-Peptide Cyclotransferase (QPCT))

Autre désignation: Glutaminyl Peptide Cyclotransferase (QPCT Produits)

Synonymes: GCT Kit ELISA, QC Kit ELISA, sQC Kit ELISA, QPCT Kit ELISA, 5730422A13Rik Kit ELISA, Qpctl1 Kit ELISA, RGD1562284 Kit ELISA, qpct Kit ELISA, glutaminyl-peptide cyclotransferase Kit ELISA, glutaminyl-peptide cyclotransferase (glutaminyl cyclase) Kit ELISA, glutaminyl-peptide cyclotransferase L homeolog Kit ELISA, QPCT Kit ELISA, Qpct Kit ELISA, M6_Spy0443 Kit ELISA, M5005_Spy_0416 Kit ELISA, M28_Spy0404 Kit ELISA, CJA_2408 Kit ELISA, CCNA_02733 Kit ELISA, Tsp_03182 Kit ELISA, qpct.L Kit ELISA

Sujet:

Gene Name: Glutaminyl Peptide Cyclotransferase

Gene Aliases: GCT, QC, EC, Glutaminyl Cyclase, Glutaminyl-tRNA cyclotransferase

ID gène: 25797

UniProt: Q16769