Glutathione Synthetase Kit ELISA

N° du produit ABIN5654726

Détail du produit Glutathione Synthetase Kit ELISA

Antigène: Glutathione Synthetase (GSS)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutathione Synthetase (GSS). No significant cross-reactivity or interference between Glutathione Synthetase (GSS) and analogues was observed.

Sensibilité: 0.33 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Synthetase (GSS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Synthetase (GSS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Synthetase (GSS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Synthetase (GSS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Synthetase (GSS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Synthetase (GSS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glutathione Synthetase (GSS)

Autre désignation: Glutathione Synthetase (GSS Produits)

Synonymes: PRNP Kit ELISA, 6835 Kit ELISA, CG32497 Kit ELISA, CG33065 Kit ELISA, CG6835 Kit ELISA, Dmel\\CG6835 Kit ELISA, F21A20.90 Kit ELISA, F21A20_90 Kit ELISA, GLUTATHIONE SYNTHETASE Kit ELISA, GSHB Kit ELISA, glutathione synthetase 2 Kit ELISA, GSHS Kit ELISA, AI314904 Kit ELISA, GS-A/GS-B Kit ELISA, gssls-a Kit ELISA, glutathione synthetase Kit ELISA, Glutathione Synthetase Kit ELISA, glutathione synthetase 2 Kit ELISA, glutathione synthetase L homeolog Kit ELISA, GSS Kit ELISA, GSH2 Kit ELISA, Tola_1250 Kit ELISA, PC1_3711 Kit ELISA, Dd586_3633 Kit ELISA, Kvar_0701 Kit ELISA, Alvin_0197 Kit ELISA, Nhal_0215 Kit ELISA, BC1002_0254 Kit ELISA, Cseg_4017 Kit ELISA, Snov_3844 Kit ELISA, Aazo_1904 Kit ELISA, M301_2613 Kit ELISA, Bresu_0515 Kit ELISA, Isop_1460 Kit ELISA, Marme_4020 Kit ELISA, Thicy_0325 Kit ELISA, Sinme_3668 Kit ELISA, Mesop_0757 Kit ELISA, Gss Kit ELISA, gss.L Kit ELISA

Sujet:

Gene Name: Glutathione Synthetase

Gene Aliases: Glutathione synthase

ID gène: 25458

UniProt: P46413

Pathways: L'effet Warburg