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CD42b Kit ELISA

GP1BA Reactivité: Humain Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma
N° du produit ABIN5654764
  • Antigène Voir toutes CD42b (GP1BA) Kits ELISA
    CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))
    Reactivité
    • 4
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.781 ng/mL - 50 ng/mL
    Seuil minimal de détection
    0.781 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba). No significant cross-reactivity or interference between Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba) and analogues was observed.
    Sensibilité
    0.35 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CD42b (GP1BA) Kits ELISA
    CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))
    Autre désignation
    Glycoprotein Ib Alpha Polypeptide, Platelet (GP1BA Produits)
    Synonymes
    BDPLT1 Kit ELISA, BDPLT3 Kit ELISA, BSS Kit ELISA, CD42B Kit ELISA, CD42b-alpha Kit ELISA, DBPLT3 Kit ELISA, GP1B Kit ELISA, GPIbA Kit ELISA, VWDP Kit ELISA, GP1BA Kit ELISA, GPIba Kit ELISA, GPIbalpha Kit ELISA, glycoprotein Ib platelet alpha subunit Kit ELISA, glycoprotein Ib (platelet), alpha polypeptide Kit ELISA, glycoprotein 1b, alpha polypeptide Kit ELISA, GP1BA Kit ELISA, Gp1ba Kit ELISA
    Sujet

    Gene Name: Glycoprotein Ib Alpha Polypeptide, Platelet

    Gene Aliases: CD42b, CD42-B, CD42b-Alpha, GP1-BA, BSS, GP1B, GPIBA, Glycocalicin

    ID gène
    2811
    UniProt
    P07359
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