GLO1 Kit ELISA

N° du produit ABIN5654775

Détail du produit GLO1 Kit ELISA

Antigène: GLO1 (Glyoxalase I (GLO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glyoxalase I (GLO1). No significant cross-reactivity or interference between Glyoxalase I (GLO1) and analogues was observed.

Sensibilité: 0.59 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyoxalase I (GLO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyoxalase I (GLO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyoxalase I (GLO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyoxalase I (GLO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyoxalase I (GLO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyoxalase I (GLO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GLO1 (Glyoxalase I (GLO1))

Autre désignation: Glyoxalase I (GLO1 Produits)

Synonymes: GLOD1 Kit ELISA, GLYI Kit ELISA, 0610009E22Rik Kit ELISA, 1110008E19Rik Kit ELISA, 2510049H23Rik Kit ELISA, AW550643 Kit ELISA, GLY1 Kit ELISA, Glo-1 Kit ELISA, Glo-1r Kit ELISA, Glo-1s Kit ELISA, Glo1-r Kit ELISA, Glo1-s Kit ELISA, Qglo Kit ELISA, cb554 Kit ELISA, zgc:66035 Kit ELISA, wu:fb82g09 Kit ELISA, glod1 Kit ELISA, glyi Kit ELISA, glo1 Kit ELISA, GLO1 Kit ELISA, ATGLX1 Kit ELISA, F12F1.32 Kit ELISA, F12F1_32 Kit ELISA, GLYOXALASE I Kit ELISA, glyoxalase I homolog Kit ELISA, trypanothione-dependent glyoxalase I Kit ELISA, glyoxalase I Kit ELISA, glyoxalase 1 Kit ELISA, glyoxalase 1 L homeolog Kit ELISA, glyoxalase 1 S homeolog Kit ELISA, lactoylglutathione lyase Kit ELISA, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein Kit ELISA, GLO1 Kit ELISA, Glo1 Kit ELISA, glo1 Kit ELISA, glo1.L Kit ELISA, glo1.S Kit ELISA, GST3 Kit ELISA, GLX1 Kit ELISA, STY1687 Kit ELISA, Bcen_2094 Kit ELISA, gloA Kit ELISA

Sujet:

Gene Name: Glyoxalase I

Gene Aliases: GLY-I, GLOD1, Glyoxalase Domain Containing 1, Lactoylglutathione lyase, Aldoketomutase, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase