GNRH1 Kit ELISA
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- Antigène Voir toutes GNRH1 Kits ELISA
- GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 12.35 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 12.35 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Gonadotropin Releasing Hormone (GnRH). No significant cross-reactivity or interference between Gonadotropin Releasing Hormone (GnRH) and analogues was observed.
- Sensibilité
- 5.25 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 h
- Plaque
- Pre-coated
- Protocole
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gonadotropin Releasing Hormone (GnRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gonadotropin Releasing Hormone (GnRH) and unlabeled Gonadotropin Releasing Hormone (GnRH) (Standards or samples) with the pre-coated antibody specific to Gonadotropin Releasing Hormone (GnRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes GNRH1 Kits ELISA
- GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))
- Autre désignation
- Gonadotropin Releasing Hormone (GNRH1 Produits)
- Synonymes
- GNRH Kit ELISA, GRH Kit ELISA, HH12 Kit ELISA, LHRH Kit ELISA, LNRH Kit ELISA, Gnrh Kit ELISA, Gnrh2 Kit ELISA, Lhrh1 Kit ELISA, Lnrh Kit ELISA, hpg Kit ELISA, Gnrha Kit ELISA, Lhrh Kit ELISA, Rgnrhg1 Kit ELISA, CGNRH-I Kit ELISA, GnRH-I Kit ELISA, GnRH Kit ELISA, gnrh Kit ELISA, gnrh1 Kit ELISA, grh Kit ELISA, lhrh Kit ELISA, lnrh Kit ELISA, mdGnRH Kit ELISA, mdgnrh Kit ELISA, mfGnRH Kit ELISA, gonadotropin releasing hormone 1 Kit ELISA, gonadotropin releasing hormone 1 L homeolog Kit ELISA, prepro-gonadotropin-releasing hormone Kit ELISA, GNRH1 Kit ELISA, Gnrh1 Kit ELISA, gnrh1.L Kit ELISA, gnrh1 Kit ELISA
- Sujet
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Gene Name: Gonadotropin Releasing Hormone
Gene Aliases: GNRH1, GRH, LNRH, LHRH, LH-RH I, Luliberin, Luteinizing-Hormone Releasing Hormone, Progonadoliberin-1, Gonadoliberin I, Gonadorelin, GnRH-associated peptide 1
- ID gène
- 25194
- UniProt
- P07490
- Pathways
- Hormone Activity
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