Histone H1 Kit ELISA

N° du produit ABIN5654927

Détail du produit Histone H1 Kit ELISA

Antigène: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of H1 Histone Family, Member 0 (H1F0). No significant cross-reactivity or interference between H1 Histone Family, Member 0 (H1F0) and analogues was observed.

Sensibilité: 0.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H1 Histone Family, Member 0 (H1F0). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H1 Histone Family, Member 0 (H1F0). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H1 Histone Family, Member 0 (H1F0), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H1 Histone Family, Member 0 (H1F0) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H1 Histone Family, Member 0 (H1F0) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H1 Histone Family, Member 0 (H1F0) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Autre désignation: H1 Histone Family, Member 0 (H1F0 Produits)

Synonymes: H5 Kit ELISA, h10 Kit ELISA, h1fv Kit ELISA, fb23b02 Kit ELISA, wu:fb23b02 Kit ELISA, zgc:65861 Kit ELISA, H1F0 Kit ELISA, H1fv Kit ELISA, H10 Kit ELISA, H1FV Kit ELISA, D130017D06Rik Kit ELISA, H1(0) Kit ELISA, h1(0)-1 Kit ELISA, h1f0 Kit ELISA, H1 Kit ELISA, H1 histone family member 0 Kit ELISA, H1 histone family, member 0 Kit ELISA, histone H1.0 Kit ELISA, H1 histone family member 0 S homeolog Kit ELISA, H1 histone family member 0 L homeolog Kit ELISA, histone I Kit ELISA, h1f0 Kit ELISA, H1F0 Kit ELISA, LOC698693 Kit ELISA, H1f0 Kit ELISA, h1f0.S Kit ELISA, h1f0.L Kit ELISA, his1 Kit ELISA

Sujet:

Gene Name: H1 Histone Family, Member 0

Gene Aliases: H1FV, H10