H2AFX Kit ELISA

N° du produit ABIN5654929

Détail du produit H2AFX Kit ELISA

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member X (H2AFX). No significant cross-reactivity or interference between H2A Histone Family, Member X (H2AFX) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member X (H2AFX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member X (H2AFX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member X (H2AFX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H2A Histone Family, Member X (H2AFX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H2A Histone Family, Member X (H2AFX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H2A Histone Family, Member X (H2AFX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: H2A Histone Family, Member X (H2AFX Produits)

Synonymes: H2A.X Kit ELISA, H2A/X Kit ELISA, H2AX Kit ELISA, AW228881 Kit ELISA, H2ax Kit ELISA, Hist5-2ax Kit ELISA, gammaH2ax Kit ELISA, zgc:56329 Kit ELISA, h2a.x Kit ELISA, h2a/x Kit ELISA, h2ax Kit ELISA, RGD1566119 Kit ELISA, h2a Kit ELISA, h2afx Kit ELISA, H2A histone family member X Kit ELISA, H2A histone family, member X Kit ELISA, H2A histone family member X L homeolog Kit ELISA, histone cluster 1, H2ah Kit ELISA, histone cluster 2, H2ab S homeolog Kit ELISA, histone H2AX Kit ELISA, H2AFX Kit ELISA, H2afx Kit ELISA, h2afx Kit ELISA, h2afx.L Kit ELISA, HIST1H2AH Kit ELISA, hist2h2ab.S Kit ELISA, LOC100522201 Kit ELISA, LOC100720536 Kit ELISA

Sujet:

Gene Name: H2A Histone Family, Member X

Gene Aliases: H2AX

ID gène: 3014

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus