SERPINH1 Kit ELISA

N° du produit ABIN5654974

Détail du produit SERPINH1 Kit ELISA

Antigène: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125.00 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125.00 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 47 (HSP47). No significant cross-reactivity or interference between Heat Shock Protein 47 (HSP47) and analogues was observed.

Sensibilité: 50 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 47 (HSP47). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 47 (HSP47). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 47 (HSP47), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 47 (HSP47) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 47 (HSP47) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 47 (HSP47) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Autre désignation: Heat Shock Protein 47 (SERPINH1 Produits)

Synonymes: AsTP3 Kit ELISA, CBP1 Kit ELISA, CBP2 Kit ELISA, HSP47 Kit ELISA, OI10 Kit ELISA, PPROM Kit ELISA, RA-A47 Kit ELISA, SERPINH2 Kit ELISA, gp46 Kit ELISA, SERPINH1 Kit ELISA, DKFZp468B197 Kit ELISA, cb216 Kit ELISA, hsp Kit ELISA, hsp47 Kit ELISA, id:ibd5060 Kit ELISA, wu:fc56b02 Kit ELISA, BERF-1 Kit ELISA, Cbp1 Kit ELISA, Cbp2 Kit ELISA, Hsp47 Kit ELISA, J6 Kit ELISA, Serpinh2 Kit ELISA, colligin Kit ELISA, astp3 Kit ELISA, cbp1 Kit ELISA, cbp2 Kit ELISA, pig14 Kit ELISA, pprom Kit ELISA, ra-a47 Kit ELISA, serpinh2 Kit ELISA, serpin family H member 1 Kit ELISA, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1b Kit ELISA, serpin H1 Kit ELISA, selenoprotein K Kit ELISA, serine (or cysteine) peptidase inhibitor, clade H, member 1 Kit ELISA, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) L homeolog Kit ELISA, SERPINH1 Kit ELISA, serpinh1b Kit ELISA, LOC100009353 Kit ELISA, Selenok Kit ELISA, Serpinh1 Kit ELISA, serpinh1.L Kit ELISA

Classe de substances: Viral Protein

Sujet:

Gene Name: Heat Shock Protein 47

Gene Aliases: CBP2, AsTP3, CBP1, PIG14, RA-A47, SERPINH1, SERPINH2, Colligen, Gp46, Serpin Peptidase Inhibitor Clade H Member 1, Heat Shock Protein 47, Collagen Binding Protein 2