HSP90AA1 Kit ELISA

N° du produit ABIN5654979

Détail du produit HSP90AA1 Kit ELISA

Antigène: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1). No significant cross-reactivity or interference between Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1) and analogues was observed.

Sensibilité: 0.65 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90 kDa Alpha A1 (HSP90aA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Autre désignation: Heat Shock Protein 90kDa Alpha A1 (HSP90AA1 Produits)

Synonymes: EL52 Kit ELISA, HSP86 Kit ELISA, HSP89A Kit ELISA, HSP90A Kit ELISA, HSP90N Kit ELISA, HSPC1 Kit ELISA, HSPCA Kit ELISA, HSPCAL1 Kit ELISA, HSPCAL4 Kit ELISA, HSPN Kit ELISA, Hsp89 Kit ELISA, Hsp90 Kit ELISA, LAP2 Kit ELISA, Hsp86 Kit ELISA, Hspca Kit ELISA, htpG Kit ELISA, 86kDa Kit ELISA, 89kDa Kit ELISA, AL024080 Kit ELISA, AL024147 Kit ELISA, Hsp86-1 Kit ELISA, hsp4 Kit ELISA, HSP90 Kit ELISA, HSP90AA1 Kit ELISA, fb17b01 Kit ELISA, hsp90 Kit ELISA, hsp90a Kit ELISA, hsp90a.1 Kit ELISA, hsp90alpha Kit ELISA, wu:fb17b01 Kit ELISA, zgc:86652 Kit ELISA, Hsp90alpha Kit ELISA, heat shock protein 90 alpha family class A member 1 Kit ELISA, heat shock protein 90, alpha (cytosolic), class A member 1 Kit ELISA, Heat Shock Protein 90, cytosolic Kit ELISA, heat shock protein 90A Kit ELISA, molecular chaperone Kit ELISA, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 Kit ELISA, heat shock protein HSP 90-alpha Kit ELISA, heat shock protein 90kDa alpha (cytosolic), class A member 1 Kit ELISA, HSP90AA1 Kit ELISA, Hsp90aa1 Kit ELISA, HSP90A Kit ELISA, hsp90A Kit ELISA, hsp90aa1.1 Kit ELISA, LOC108698781 Kit ELISA

Sujet:

Gene Name: Heat Shock Protein 90 kDa Alpha A1

Gene Aliases: HSP86, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals