HDLBP Kit ELISA

N° du produit ABIN5655125

Détail du produit HDLBP Kit ELISA

Antigène: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of High Density Lipoprotein Binding Protein (HDLBP). No significant cross-reactivity or interference between High Density Lipoprotein Binding Protein (HDLBP) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Autre désignation: High Density Lipoprotein Binding Protein (HDLBP Produits)

Synonymes: HBP Kit ELISA, PRO2900 Kit ELISA, VGL Kit ELISA, 1110005P14Rik Kit ELISA, AA960365 Kit ELISA, AI118566 Kit ELISA, D1Ertd101e Kit ELISA, HDLBP Kit ELISA, vigilin Kit ELISA, cb591 Kit ELISA, cb811 Kit ELISA, hdlbp Kit ELISA, wu:fb36d01 Kit ELISA, wu:fb94h08 Kit ELISA, wu:fc23d06 Kit ELISA, wu:fc75h07 Kit ELISA, high density lipoprotein binding protein Kit ELISA, high density lipoprotein (HDL) binding protein Kit ELISA, vigilin Kit ELISA, high density lipoprotein binding protein (vigilin) S homeolog Kit ELISA, high density lipoprotein binding protein a Kit ELISA, HDLBP Kit ELISA, Hdlbp Kit ELISA, hdlbp.S Kit ELISA, hdlbpa Kit ELISA

Sujet:

Gene Name: High Density Lipoprotein Binding Protein

Gene Aliases: HBP, VGL, Vigilin

ID gène: 3069

UniProt: Q00341