HAL Kit ELISA

N° du produit ABIN5655153

Détail du produit HAL Kit ELISA

Antigène: HAL (Histidine Ammonia-Lyase (HAL))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Histidine Ammonia Lyase (HAL). No significant cross-reactivity or interference between Histidine Ammonia Lyase (HAL) and analogues was observed.

Sensibilité: 0.63 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Ammonia Lyase (HAL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Ammonia Lyase (HAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Ammonia Lyase (HAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Ammonia Lyase (HAL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histidine Ammonia Lyase (HAL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histidine Ammonia Lyase (HAL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HAL (Histidine Ammonia-Lyase (HAL))

Autre désignation: Histidine Ammonia Lyase (HAL Produits)

Synonymes: HIS Kit ELISA, HSTD Kit ELISA, hal Kit ELISA, hstd Kit ELISA, xhal Kit ELISA, HAL Kit ELISA, BA3712 Kit ELISA, DDBDRAFT_0187742 Kit ELISA, DDBDRAFT_0231727 Kit ELISA, DDB_0187742 Kit ELISA, DDB_0231727 Kit ELISA, zgc:136980 Kit ELISA, his Kit ELISA, Hsd Kit ELISA, histidase Kit ELISA, histidine ammonia-lyase Kit ELISA, histidine ammonia-lyase, gene 1 L homeolog Kit ELISA, histidine ammonia-lyase HutH Kit ELISA, histidine ammonia-lyase, gene 1 Kit ELISA, histidine ammonia lyase Kit ELISA, HAL Kit ELISA, hal.1.L Kit ELISA, hutH Kit ELISA, Tc00.1047053506247.220 Kit ELISA, Plav_1493 Kit ELISA, hal Kit ELISA, hal.1 Kit ELISA, Hal Kit ELISA

Sujet:

Gene Name: Histidine Ammonia Lyase

Gene Aliases: HIS, Histidase