HDAC4 Kit ELISA

N° du produit ABIN5655173

Détail du produit HDAC4 Kit ELISA

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 4 (HDAC4). No significant cross-reactivity or interference between Histone Deacetylase 4 (HDAC4) and analogues was observed.

Sensibilité: 0.129 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 4 (HDAC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 4 (HDAC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 4 (HDAC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 4 (HDAC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 4 (HDAC4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 4 (HDAC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Autre désignation: Histone Deacetylase 4 (HDAC4 Produits)

Synonymes: CG1770 Kit ELISA, DHDAC4 Kit ELISA, Dmel\\CG1770 Kit ELISA, GC1770 Kit ELISA, HDAC Kit ELISA, HDAC4a Kit ELISA, dHDAC4 Kit ELISA, dmHDA405 Kit ELISA, hdac4 Kit ELISA, dana_GLEANR_19966 Kit ELISA, DanaGF18710 Kit ELISA, GF18710 Kit ELISA, HDAC4 Kit ELISA, AHO3 Kit ELISA, BDMR Kit ELISA, HA6116 Kit ELISA, HD4 Kit ELISA, HDAC-4 Kit ELISA, HDAC-A Kit ELISA, HDACA Kit ELISA, 4932408F19Rik Kit ELISA, AI047285 Kit ELISA, wu:fa96d08 Kit ELISA, wu:fc56f08 Kit ELISA, zgc:152701 Kit ELISA, histone deacetylase 4 Kit ELISA, hypothetical protein Kit ELISA, Histone deacetylase 4 Kit ELISA, GF18710 gene product from transcript GF18710-RC Kit ELISA, PTRG_03699 Kit ELISA, PGTG_15512 Kit ELISA, HDAC4 Kit ELISA, Dana\GF18710 Kit ELISA, Hdac4 Kit ELISA, hdac4 Kit ELISA

Sujet:

Gene Name: Histone Deacetylase 4

Gene Aliases: HD4, HDAC-A, HDACA

ID gène: 208727

UniProt: Q6NZM9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process