Histone H2A Kit ELISA

N° du produit ABIN5655179

Détail du produit Histone H2A Kit ELISA

Antigène: Histone H2A

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Histone H2A (H2A). No significant cross-reactivity or interference between Histone H2A (H2A) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2A (H2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H2A (H2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2A (H2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2A (H2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H2A (H2A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H2A (H2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Histone H2A

Abstract: Histone H2A Produits

Synonymes: 5499 Kit ELISA, CG5499 Kit ELISA, Dmel\\CG5499 Kit ELISA, H2A Kit ELISA, H2A.F/Z Kit ELISA, H2A.X Kit ELISA, H2A.Z Kit ELISA, H2AV Kit ELISA, H2AV_DROM Kit ELISA, H2AX Kit ELISA, H2AZ Kit ELISA, H2Av Kit ELISA, H2AvD Kit ELISA, H2a.V Kit ELISA, H2av Kit ELISA, H2avD Kit ELISA, HIS Kit ELISA, HIS2AV Kit ELISA, HIS2AVD Kit ELISA, His Kit ELISA, His2 Kit ELISA, His2AV Kit ELISA, His2AvD Kit ELISA, HisH2Av Kit ELISA, Hist Kit ELISA, Hist2av Kit ELISA, gamma-H2Av Kit ELISA, gamma-HIS2AV Kit ELISA, gamma-His2Av Kit ELISA, gammaH2Av Kit ELISA, h2AvD Kit ELISA, his Kit ELISA, l(3)05146 Kit ELISA, l(3)810 Kit ELISA, l(3)97Dd Kit ELISA, l(3)L1602 Kit ELISA, DDBDRAFT_0205974 Kit ELISA, DDBDRAFT_0216271 Kit ELISA, DDB_0205974 Kit ELISA, DDB_0216271 Kit ELISA, RGD1564767 Kit ELISA, H2a-221 Kit ELISA, H2A-III Kit ELISA, HIST1H2AA Kit ELISA, Histone H2A variant Kit ELISA, putative histone H2A Kit ELISA, histone H2A Kit ELISA, histone cluster 1 H2A family member F Kit ELISA, histone H2A-like Kit ELISA, histone cluster 1, H2af Kit ELISA, histone2A1 Kit ELISA, histone cluster 1, H2A, III Kit ELISA, histone cluster 1, H2ac Kit ELISA, His2Av Kit ELISA, LMJF_17_0280 Kit ELISA, H2AX Kit ELISA, Hist1h2af Kit ELISA, LOC100425378 Kit ELISA, his2a1 Kit ELISA, HIST1H2A3 Kit ELISA, HIST1H2AC Kit ELISA, h2a-A Kit ELISA

Sujet:

Gene Name: Histone H2A