HGD Kit ELISA

N° du produit ABIN5655196

Détail du produit HGD Kit ELISA

Antigène: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Homogentisate-1,2-Dioxygenase (HGD). No significant cross-reactivity or interference between Homogentisate-1,2-Dioxygenase (HGD) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homogentisate-1,2-Dioxygenase (HGD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homogentisate-1,2-Dioxygenase (HGD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homogentisate-1,2-Dioxygenase (HGD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homogentisate-1,2-Dioxygenase (HGD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Autre désignation: Homogentisate-1,2-Dioxygenase (HGD Produits)

Synonymes: CG4779 Kit ELISA, Dmel\\CG4779 Kit ELISA, PSPTO3551 Kit ELISA, DDBDRAFT_0185702 Kit ELISA, DDBDRAFT_0191461 Kit ELISA, DDB_0185702 Kit ELISA, DDB_0191461 Kit ELISA, Hgo Kit ELISA, aku Kit ELISA, hgo Kit ELISA, AKU Kit ELISA, HGO Kit ELISA, homogentisate 1,2-dioxygenase Kit ELISA, Homogentisate 1,2-dioxygenase Kit ELISA, homogentisate 1, 2-dioxygenase Kit ELISA, homogentisate 1,2-dioxygenase L homeolog Kit ELISA, hgo Kit ELISA, HGD Kit ELISA, hmgA Kit ELISA, Shewana3_2481 Kit ELISA, hgd Kit ELISA, BC1002_0545 Kit ELISA, BC1003_0502 Kit ELISA, BC1001_0489 Kit ELISA, Sinme_2995 Kit ELISA, Sphch_0441 Kit ELISA, Hgd Kit ELISA, hgd.L Kit ELISA

Sujet:

Gene Name: Homogentisate-1,2-Dioxygenase

Gene Aliases: AKU, HGO, Homogentisic Acid Oxidase, Homogentisicase, Homogentisate oxygenase

ID gène: 3081

UniProt: Q93099