UBE2K Kit ELISA

N° du produit ABIN5655204

Détail du produit UBE2K Kit ELISA

Antigène: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Huntingtin Interacting Protein 2 (HIP2). No significant cross-reactivity or interference between Huntingtin Interacting Protein 2 (HIP2) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Huntingtin Interacting Protein 2 (HIP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Huntingtin Interacting Protein 2 (HIP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Huntingtin Interacting Protein 2 (HIP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Huntingtin Interacting Protein 2 (HIP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Huntingtin Interacting Protein 2 (HIP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Huntingtin Interacting Protein 2 (HIP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Autre désignation: Huntingtin Interacting Protein 2 (UBE2K Produits)

Synonymes: E2-25K Kit ELISA, HIP2 Kit ELISA, HYPG Kit ELISA, LIG Kit ELISA, UBC1 Kit ELISA, E2-25k Kit ELISA, Hip2 Kit ELISA, Hypg Kit ELISA, Lig Kit ELISA, e2-25k Kit ELISA, hip2 Kit ELISA, hypg Kit ELISA, lig Kit ELISA, AW492011 Kit ELISA, D5Ertd601e Kit ELISA, ube2k Kit ELISA, zgc:103472 Kit ELISA, zgc:110791 Kit ELISA, ubiquitin conjugating enzyme E2 K Kit ELISA, ubiquitin-conjugating enzyme E2K Kit ELISA, ubiquitin conjugating enzyme E2 K S homeolog Kit ELISA, ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) Kit ELISA, ubiquitin-conjugating enzyme E2Ka (UBC1 homolog, yeast) Kit ELISA, UBE2K Kit ELISA, Ube2k Kit ELISA, ube2k.S Kit ELISA, ube2k Kit ELISA, ube2kb Kit ELISA, ube2ka Kit ELISA

Sujet:

Gene Name: Huntingtin Interacting Protein 2

Gene Aliases: HYPG, LIG, UBE2K, Ubiquitin-Conjugating Enzyme E2K, Ubiquitin carrier protein, Ubiquitin-protein ligase

ID gène: 3093

UniProt: P61086